PNA-FISH

Abbreviation for "Peptide Nucleic Acid - Fluorescence In Situ Hybridisation". A peptide is an organic chemical compound resulting from the linkage of several amino acids arranged in a defined order (sequence). It is a medical-diagnostic analytical method with which certain DNA and RNA sequences can be localised in tissues, cells, cell nuclei and chromosomes in minute quantities. An artificially produced probe made of a nucleic acid is used, which "hybridises" the nucleic acid to be detected, i.e. binds it and makes it visible. The term "in situ" refers to the fact that the detection is carried out directly in the respective structure and not, for example, biochemically in a test tube. The PNA probes used are marked with a fluorescent dye. The PNA-FISH method is mainly used in medicine to detect and identify pathogens in human blood.

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PNA-FISH

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